24 Suitability of the methods towards the estimation of bulk drug

24 Suitability of the methods towards the estimation of bulk drug checked and found the mean recovery of 98.88 ± 0.45% this high percentage recovery proved that the method can adoptable for the estimation of TL in bulk. For the application of the proposed method to formulation the procured tablets were subjected to the analysis for their contents of TL by the proposed method and reported UV spectrophotometric method reported by Nanda et al.7 From test conducted about 99.91 and 99.67% assay was resulted with the proposed and existed method (Table 2). The results obtained are given in Table 3. The percentage relative standard deviation (% RSD) for inter, intra-day precision

was about 0.898 and 0.945 respectively which was very low and within the acceptance limits for precision experiments, PF-06463922 purchase evidencing repeatability

(precision) of the method. The resulted recovery at three levels was with the % RSD of 0.94–0.98% for TL (Table 3). The above % RSD were found within the acceptance limit for accuracy of <2% RSD this good accuracy of the purposed method. The effect of the MO was studied by measuring the absorbance of solutions containing TL (10 μg mL−1), and 0.5 mL of MO solution at various Selleckchem Doxorubicin concentration (0.025–0.15% wt/v). The results are portrayed in Fig. 5. As MO concentration of 0.05% wt/v gave a maximum absorbance. Results of quantity of MO to be added is given in Fig. 6. From the results it was established that Fossariinae 0.05 mL of 0.05% wt/v MO is sufficient to make complex with maximum absorbance. Volumes of above 0.05 mL reagent had no marked effect on the chromogen formation. The studied excipients

do not cause any interference in the estimation of the drug (Table 4). Likewise the placebo mixture of above excipients was prepared without the drug and studied at the wavelength of estimation for determining any absorbance for the chloroform extractable material in the placebo. Yellow color was not developed in the extract revealed the selectivity of the present method. Likewise the results of stability form the shown from Fig. 7 evidenced that the chromogen was stable more than 3.5 h. The results obtained were within the suggested limits for % RSD (<2%) (Table 5). Ruggedness was established by determining TL in the tablet formulation using two different spectrophotometer Shimadzu UV mini-1240 (system I) and SCINCO, Neosys-2000 DRS-UV provided with liquid sample analysis port (system II) and two different analysts (I and II). The results obtained were within the recommended % RSD limit (<2%) (Table 5). The proposed ion-pair extractive colorimetric estimation of tolterodine tartrate (TL) in bulk and in formulation is more sensitive, specific (selective), rapid and cost effective. The highest % recovery of the method proved that the present method was more accurate and comparable with that of reference method.

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