Recently, NS5A replication
complex inhibitors were developing and clinical trials revealed drug associated resistance variant (RAV) such as L31M and Y93H. Thus, the NS5A polymorphisms of NS5A regions will play an important role but the little is known. The aim of this study is to evaluate the clinical impact of NS5A polymorphisms in patients with HCV genotype 1b. Methods: Twenty three treatment naïve patients with chronic hepatitis C genotype 1b were enrolled. There were 13 men and 10 women (mean age, 54.5 ± 11.7 years). The NS5A regions (aa 2209-2248; ISDR and aa 2334-2379; AZD1208 cell line IRRDR) were examined by direct sequencing. Sequences of the HCVJ strain were defined as the proto-type. Results: Two of 23 (8.6%) patients had RAV to NS5A inhibitors. The variants are Q54H (n = 6), Y93H (n = 1) L31M + Q54H (n = 1), Q54H + Q62E (n = 1). The sequence of the HCVJ strain were defined as the consensus sequence and the approach of counting the number of mutations to the chosen consensus sequence for ISDR and IRRDR. The number of ISDR mutations was none (n = 6), 1 (n = 7), 2 (n = 6), Selleck XL765 3 (n = 3), 4 (n = 1) and for IRRDR, 3 (n = 4), 4 (n = 6), 5 (n = 2), 6 (n = 37), 7 (n = 2), 8 (n = 2). There
are no association between ISDR and IRRDR. We also cannot find the relationship between NS5A RAV with ISDR and IRRDR Conclusion: HCV NS5A polymorphisms in patients with HCV genotype 1b is widely variety and the variants such as selleck inhibitor NS5A RAV, ISDR and IRRDR were independent. Key Word(s): 1. HCV IFN NS5A Presenting Author: MIE SHINOHARA Additional Authors: ISHII KOJI, KOGAME MICHIO, NORITAKA WAKUI, TAKASHI IKEHARA, SHINOHARA MASAO, HIDENARI NAGAI, MANABU WATANABE, YOSHIHIRO IGARASHI, YASUKIYO SUMINO Corresponding Author: MIE SHINOHARA Affiliations: Tokyo Kamata Medical Center, Toho University Medical Center, Toho University Medical
Center, Toho University Medical Center, Toho University Medical Center, Toho University Medical Center, Toho University Medical Center, Toho University Medical Center, Toho University Medical Center Objective: The molar concentration ratio of branched-chain amino acids (BCAA) to tyrosine (BTR) in serum decreases with severity of liver diseases such as chronic hepatitis C (CHC). In addition, serum levels of tyrosine (Tyr) are known to increase in patients with liver cirrhosis. However, it is unclear whether these parameters change after hepatitis C virus (HCV) is eradicated in CHC patients treated with interferon (IFN)-based therapy. The aim of this study was to clarify whether serum BTR, BCAA and Tyr change in response to IFN-based therapy in association with liver histological findings.