Measured in the cell w Ssigbar during the period of 75 seconds and negligible before. However, more than two-thirds Mubritinib 366017-09-6 of the GFP signal VATM in this interval was 75 seconds away, as shown in the graph. Perkin Elmer Ultraview microscope. Found at: doi: 10.1371/journal.pone.0008585.s001 Figure S2 phagosome increased HTES volume and dilution of the fluid phase marker before premature exocytosis. The cell with TRITC-dextran and yeast incubated for three hours, then the medium was replaced with buffer, the cells were applies with a thin layer of agarose, which was dried slightly to induce premature exocytosis covered, and the sample both. All endosomes, macropinosomes but should contain new TRITC-dextran. The red pixel intensity of t increases with particle phagosome gr Than a few grams ere he, Indicating an influx of unlabeled fluid.
A vacuole separates the phagosome and f Carried into the cell, and yeast in the first multipartikul Re is phagosome exocytosis. Separation and recycling of TRITC-dextran concentrate in the vacuole, so that the pixel intensity t again, k The internal vesicles in the vacuole can be seen to 477 seconds. Zeiss LSM510 microscope. Found at: doi: Figure MK-2206 Akt inhibitor S3 10.1371/journal.pone.0008585.s002 Erh hte volume on strength of the phagosome and the dilution of the fluid phase marker before premature exocytosis. This sample was prepared as described in the exception that the cells were in the buffer for 30 minutes left described before visualization. Sun sp T, but not early endosomes should contain TRITC-dextran.
The results were Similar to those of the first experiment, n Namely the expansion of the phagosome and the dilution of TRITC-dextran, the separation of a vacuole, the yeast exocytosis, and an increase Increase the concentration of TRITC-dextran, that the volume the vacuole at the sorting is reduced. Zeiss LSM510 microscope. Found at: doi: Figure S4 10.1371/journal.pone.0008585.s003 expansion and separation phagosome vacuole in a cell expressing GFP dajumin contractile vacuole markers. The cells were GFP and MRFP dajumin GE Cares, they were mixed with yeast tt 2 hours. The cells were covered with a thin layer of agarose, which was slightly dried to a premature induce exocytosis covered. A vacuole separates the phagosome and moves from a tail of actin filaments. W During the phagosome is the exocytosis.
N dajumin GFP label incorporated into the membrane of the vacuole or phagosome, said the contractile vacuole system is not the source of the membrane and liquid absorbed. Zeiss LSM510 microscope. Bar, 5 mm. Found at: doi: Movie S1 10.1371/journal.pone.0008585.s004 delivery of V-ATPase in a new phagosome. The cells expressing GFP and MRFP VATM whitewashed. It has been phagocytosed a yeast cell alive. Found at: doi: Movie S2 10.1371/journal.pone.0008585.s005 delivery of V-ATPase in a new phagosome. The cell is expressing GFP was VATM and with TRITC-dextran label was incubated on endosomes. The absorption of heat killed yet shown ended yeast. Found at: doi: 10.1371/journal.pone.0008585.s006 Movie S3 GFP labeling of macropinosomes with 2FYVE, a biosensor for PIP. The cells expressing GFP and MRFP 2FYVE is whitewashed.
The resulting macropinosomes is first labeled with the actin marker. Actin disappear T and GFP 2FYVE link starts after about a minute. It consists of an additional 2 to 3 minutes on macropinosomes and fragments of behavior that goes with the first or sorting endosome. Found at: doi: Movie S4 10.1371/journal.pone.0008585.s007 labeling of GFP 2FYVE of phagosomes with bacteria. The cell is eaten by the CFP and 2FYVE MRFP lime and bacteria. New phagosomes from the first marker and actin by GFP 2FYVE about a minute sp Ter and maintaining selected several minutes. Fusion and fission events characteristic of early phagosomes may need during the period 2FYVE to see GFP labeling. Found at: doi: 10.1371/journal.pone.0008585.s008 movie S5 L mixture of ATPase V m