Locked GE with fulvestrant without Sch To that induced by neomycin showed no significant Ver Change in the number of GFP / BrdUcells compared to controls. In fish treated with neomycin and allowed to recover the vehicle only for 24 hours, we see a lot of new hair cells, GFP. Among these cells are the most BrdU, indicating that they arise from the proliferation of supporting cells. In the groups treated flubendazole and topotecan, get up the hair cells are less and virtually none of these hair cells are labeled with BrdU. The number of hair cells GFP / BrdU is consistent with the number of immature cells neomycin, hair expected insensitive. In particular, there is a significant decrease in support cells GFP / BrdU. We do not observe repopulation of hair cells, the BrdU-GFP in the presence of mitotic inhibitors after treatment with neomycin. Fulvestrant in the treatment groups, most cells after 24 h and BrdU GFPhair regenerated Similar procedures L groups. There is a slight decrease in the proliferation of supporting cells, as less GFP / BrdU cells. Thus, the slight decrease or a delay Storage at the regeneration of hair cells in the larvae treated with fulvestrant be attributed to a moderate decrease in cell division support. The k of fulvestrant on the number of hair cells Can only behind the 24 hour time. To determine whether the inhibitors are clearly toxic to dividing cells no support within the neuromasts total number of cells were performed for each neuromast. The fish were exposed to inhibitors for 48 hours without pretreatment with neomycin and then End labeled with SYTOX Green nucleic Acid stain. Examination by confocal microscopy showed a high mag AREA without condensation and DNA fragmentation is evident in the cells of the neuromasts in each treatment group or a contr On, there was no evidence of significant gaps in the missing cells. In particular, you should Dopamine Receptor group with drugs that inhibit the lack of exposure to neomycin treated first cells were not significantly less likely than their sham-controlled exhibit The vehicle treated.
Thissuggests such as topotecan, flubendazole, fulvestrant caused and no cell death not supported distribution of the concentrations either alone or w Used during the 48 h after treatment with neomycin. Recovery from inhibition W During flubendazole and topotecan seems to be the regeneration of hair cells by affecting mitotically active precursor Shore cells intended to prevent lkern with neuromast hair cells back to bev, It is not clear whether these drugs to stop cells in the cell cycle or get tet precursors. To determine whether precursors k Can recover from inhibitor treatment, we exposed fish inhibitors 24 h treatment with neomycin following. We then thoroughly rinsed and allowed the fish to recover an additional keeping 72 h. Hair cell counts were performed after 1, 24, 48, 72 and 96 h after initiation of treatment with neomycin. Regeneration exhibition fight against larvae of robust hair cells with numbers that refer to a pre-treatment levels between 72 and 96 h after treatment. The incubation with 24-hour flubendazole first production Bl skirts of hair cells, but regeneration takes strong inhibitor after washing with a rate Similar contr L Fish, although zinc Siege. In contrast, topotecan has lasting effect on regeneration. As expected, no new hair cells produced in the fish treate.