The were compared with those of levofloxacin and ciprofloxacin as well as those of trovafloxacin as an example of leistungsf HIGEN and broad-spectrum fluoroquinolone compared. In addition isolated the F Ability of MLN8054 ABT 492 in forming cleavable complexes studied aureus with DNA gyrase and topoisomerase IV from E. coli and S.. Materials and Methods antibiotics. ABT 492 was prepared by the Wakunaga Pharmaceutical Co, Hiroshima, Japan. Trovafloxacin, levofloxacin and ciprofloxacin were from Abbott Laboratories, Abbott Park, Ill. Erythromycin, clindamycin, penicillin and powders reference made to vancomycin from the Convention of the United States Pharmacopeial Convention, Inc., Rockville purchased, Md. Ampicillin was obtained from Sigma Chemical Company, St .
Louis, MO Determination of the antibacterial activity of t bought. The bacterial strains mme For sensitivity AZD1152-HQPA Tsstudien be used are either clinical isolates from Abbott Laboratories culture of the authors. Mailing Address: R47T, AP52, Abbott Laboratories, Abbott Park, IL 60 064 3537th Phone: 937 7706th Fax: 935 0400th E mail: @ angela.nilius abbott.com. � Present address: Focus Technologies, Herndon, VA 20171st 3260 collection strains or Referenzst From the American Type Culture Collection. Susceptibility Th of aerobic, non-demanding species, S. pneumoniae and H. influenzae were visually determined by microdilution as described by the National Committee for Clinical Laboratory Standards described.
Sensitivities of Neisseria gonorrhoeae and Helicobacter pylori were determined by agar dilution method as described by the contr The NCCLS quality of t, as indicated by the NCCLS, was performed for all tests, the NCCLS reference methods used, our results were in accordance with NCCLS standards. Sensitivities were also in a medium containing rat serum 50% serum or the man who W rme For 30 min inactivated at 56 had intended. The minimum bactericidal concentrations were determined by microdilution in conjunction with MIC NCCLS methods. Sensitivities of Legionella spp. were determined on charcoal-yeast extract agar buffered with incubation at 35 in an atmosphere re with 5% CO 2. Mycobacterium avium were sensitivities on agar with 0.5% glycerol, and 7:10 10% Ls Acid albumin dextrose catalase enrichment by incubation at 35 in an atmosphere with 5% CO 2 re erg Determined complements.
Susceptibility were Th of anaerobic species determined by dilution with agar-agar with Wilkins Chalgren incubation at 35 in an oxygen free atmosphere re with more than 4% CO 2. The sensitivity of Mycoplasma pneumoniae was by microdilution SP4 medium with incubation at 35 in the Au Enluft determined. The sensitivity was determined by Borrelia burgdorferi broth macrodilution in Barbour Stoenner Kelly medium with an incubation at 35 in an atmosphere re with 5% CO 2. The sensitivity was trachomatis Chlamydia trachomatis by microdilution with infected McCoy cells at 37 in an atmosphere with 5% CO 2 re for 48 h, intracellular Re C incubation was determined by F Fluorescentantibody dyeing. Determination of the molecular mechanisms of resistance. The molecular mechanisms of macrolide and vancomycin resistance in some Gram-positive isolates were identified by PCR amplification of the Mef efflux, Erm methylase, VanA, and VanB genes identified as described above. Lactamase was determined by nitrocefin hydrolysis. Mutations in the quinolone res