The chambers were then incubated for 24 hours at 37 °C in a humid atmosphere of 5% CO2. After incubation, the number of cells that migrated to the lower chamber was determined with eosin staining. The cells entered the substrate in the lower chamber and then were mixed uniformly. At last, we counted the cells under the microscope (10 randomly selected high power fields)

individually. Statistical Analysis Data were analyzed with SPSS 11.5 software. Statistics processing about clinical data were evaluated INK128 with χ 2 test, Spearman’s rank correlation test. Statistics processing about in vitro experimentation were t test and ANOVA. P < 0.05 was considered significant and P < 0.001 highly significant in all statistical analyses. Results Immunohistochemical Staining of CCR7, MMP-9, and MMP-2 (Table 1) Table 1 The chemokine receptor

expression ratios of T-NHL group and comparison group [number of cases (%)] Group n CCR7 MMP-9 MMP-2 T-NHL group 41 34 (82.9) 36 (87.8) 29 (70.7) Control group 19 3 (15.8) 3 (15.8) 2 (10.5) χ 2   32.219* 29.598* 18.845* *P < 0.01 The result for CCR7, MMP-9, and MMP-2 revealed a predominantly cytoplasmic staining. A focal weak membrane Copanlisib mw staining (Figure 1) was observed. The high expression ratio of CCR7, MMP-9, and MMP-2 were 82.9%, 87.8%, and 70.7% in T-NHL specimens, respectively. All markers’ high expression ratios were higher than that in hyperplastic 4��8C lymph node group (P < 0.01). Figure 1 The expression of CCR7, MMP-9 and MMP-2 in T-NHL with immunohistochemical staining. These markers all express in the cytoplasm. Some yellow or brown yellow granules in

the cytoplasm are postive. The immunohistochemical staining was performed with S-P method and these photoes were taken under the high power (×400). A was CCR7 stainting. The staining intensity is strong. B was MMP-9 stainting. The staining intensity is strong. C was MMP-2 staining. The staining intensity is intermediate. Expression of all parameters in T-NHL group and correlation with clinical parameters (1) There was no significant correlation of high CCR7 expression ratio with age (87.5% >60 years vs 81.8% <=60 years), sex (87% males vs. 77.8% females) and tumor size (88.0% >3 cm vs. 75.0% <3 cm) (Table 2). The positive correlation between high CCR7 expression and multiple location dissemination was found. The CCR7 expression ratio of the multiple locations group was higher than that in the single location group (92.6% vs. 64.3%, P < 0.05). Concerning WHO classification, the high expression ratio of CCR7 also was highly significantly associated with higher tumor UIUC stages. UICC stage III and IV group had 100% high CCR7 expression compared with 75% in UICC stage I and II group(P < 0.05).