43 (P = 0.018) NS NS OHCLI 0.61 (P < 0.001)   0.42 (P = 0.002) OHREF   0.61 (P < 0.001) 0.50 (P = 0.005) OHBIA NS NS 0.50 (P = 0.005) Height NS NS 0.43 (P = 0.018) Pre-HD weight 0.43 (P = 0.017) 0.55 (P = 0.002) 0.62 (P < 0.001) Pre-HD SBP NS NS NS Pre-HD DBP 0.54 (P = 0.002) selleck NS 0.51 (P = 0.004) Pre-HD MAP 0.38 (P = 0.042) NS 0.48 (P = 0.008) NT-proANP NS NS NS LAD, LVEDD NS NS NS VCCI −0.45 (P = 0.013) NS −0.36 (P = 0.048) Cardiothoracic index

NS NS NS Vital capacity NS NS NS Pre-HD calf circumference 0.37 (P = 0.041) 0.47 (P = 0.009) 0.56 (P = 0.001) ECW 0.52 (P = 0.003) 0.50 (P = 0.005) 0.94 (P < 0.001) OH REF reference overhydration, OH CLI clinically assessed overhydration, OH BIA bioimpedance calculated overhydration, HD hemodialysis, SBP systolic blood pressure, DBP diastolic blood pressure, MAP mean arterial blood pressure,

NT-proANP N-terminal atrial natriuretic peptide, LAD left atrial diameter, LVEDD left ventricular end diastolic diameter, VCCI vena cava collapsibility index, ECW extracellular water, NS not significant Pre-HD calf circumference was positively correlated with OHREF (R = 0.37; P = 0.041), OHCLI (R = 0.47; P = 0.009), ECW/BSA (R = 0.56; P = 0.001) and pre-HD weight (R = 0.68; P < 0.001), Omipalisib mouse indicating a strong association of this simple anthropometric measure with fluid overload. VCCI was negatively correlated with OHREF (R = −0.45; P = 0.013), concordant with decreasing collapsibility of the vena cava with increasing OH. In terms of fluid overload, N-terminal

atrial natriuretic this website peptide (NT-proANP) levels did not correlate with OH or left atrial diameter (LAD) (although positively with CTI, R = 0.67; P < 0.001), parameters with known association in the normal population. The significant influence of impaired renal function on NT-proANP levels is evident by its positive correlation with serum creatinine levels (R = 0.57; P = 0.001). Blood pressure The average pre-HD BP was 125/71 mmHg, post-HD BP 110/62 mmHg, and the average BP reduction was 6/3 mmHg per one liter of OH removed. The mean 24-h ambulatory BP on a HD-free day was 116/68 mmHg. Pre-HD diastolic blood pressure (DBP) (R = 0.54; P = 0.002), mean arterial blood pressure (MAP) (R = 0.38; P = 0.04), but not systolic blood pressure (SBP) (R = 0.09; P = 0.64) correlated DOK2 with OHREF. Interestingly, after HD, SBP (R = 0.43; P = 0.02), DBP (R = 0.37; P = 0.04) and MAP (R = 0.43; P = 0.02) were positively correlated with the number of antihypertensive drugs (no correlation was seen before HD). Prognostic data are presented in the Electronic Supplementary Material. Multiple regression models Parameters significant in the univariate analyses (Table 2) were combined in multiple regression models (Table 3). Calf circumference was considered as a part of the clinical examination and not explored separately.