The membrane was then washed, blocked with 5% (wt/vol) blocking agent (non-fat skimmed milk), and incubated with a primary antibody against Omp33 obtained from mouse (INIBIC, A Coruña). Proteins were visualized by incubation with horseradish peroxidase-conjugated secondary antibody, followed by enhanced chemiluminescence ECL Plus (Amersham Pharmacia Biotech) and detected with the LAS3000 chemiluminescence detector (Fujifilm). Acknowledgements and Funding The present study was supported by grants from SERGAS (PS08/24 and
PS07/90) and INCITE 08CSA064916PR from the Xunta de Galicia, by the Spanish Network for Research in Infectious Diseases RD06/0008/0025, and by grants PI081613 and PS09/00687 PX-478 from the Instituto de Salud Carlos III (Madrid). J. Aranda is in receipt of a Berzosertib research buy Sara Borrell post-doctoral grant from the Instituto de Salud Carlos III (Madrid). M. Poza and B. Gómez are in receipt of Isidro Parga Pondal postdoctoral grants from the Xunta de Galicia. S. Rumbo and C. Rumbo are in receipt of pre-doctoral
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