2A and B). Across

5-FU datasheet treatment groups, animals spent significantly more time and distance in closed arms compared to open arms (Fig. 2A and B). In the OFT, animals treated with G-1 moved a greater distance in the center of the open field compared to those treated with vehicle or EB (Fig. 3A). G-1-treated mice also spent more time moving in the center (Fig. 3B), had a lower latency to the center (Fig. 3C), and made more entries into the center (Fig. 3D) than the Inhibitors,research,lifescience,medical EB-treated group. However, there were no differences in distance and movement time in the periphery of the OFT by treatment group (Fig. 3E and F). On the second day of OFT testing, mice in all treatment groups traveled significantly less and spent less time in the center of the open field compared to the first day of testing

(Fig. S1). Figure 2 Ovariectomized females treated chronically with either 17βE2 or G-1 were tested in the elevated plus maze. No significant effect of treatment was found in either the time spent (Fig. 2A) or distance traveled (Fig. 2B) in either … Figure 3 Ovariectomized females treated chronically with either 17βE2 or Inhibitors,research,lifescience,medical G-1 were tested in the open field arena. Mice treated chronically with G-1, but not EB, displayed a reduction in anxiety-like behaviors, traveling larger distances (cm) in the center … Protein analysis We analyzed the activation of ERK1/2 by western blotting using specific antibodies to the ERK 202/204 site; phosphorylation of this site is Inhibitors,research,lifescience,medical required for activation. We also investigated phosphorylation at the serine 118 of the ERα using

specific antibodies. Although there was no difference among treatment groups in the ventral hippocampus (Fig. 4A) in pERK levels, there was an increase in pERK in the dorsal Inhibitors,research,lifescience,medical hippocampus (Fig. 4B) in the EB-treated but not in the G-1-treated group, compared to the vehicle-treated group. In the ventral hippocampus but not in the dorsal hippocampus, there was a small decrease in Inhibitors,research,lifescience,medical total ERα in the EB-treated group compared to vehicle treatment (Fig. 5A); however, there were no differences among treatment groups in the phosphorylation of the ERK-dependent site at serine 118 (Fig. 5B) of the ERα in either the ventral or dorsal hippocampus. Figure 4 Chronic G-1 treatment does not regulate ERK activation. Animals administered EB or G-1 chronically in silastic implants were sacrificed, and western blot analysis on lysate from ventral hippocampus (A) and dorsal hippocampus (B) was all performed. pERK is … Figure 5 Although neither chronic EB nor G-1 treatment changed the phosphorylation at S118 in either the ventral hippocampus (A2) or dorsal hippocampus (B2), EB treatment decreased the ERα concentration in the ventral hippocampus (A1) but not in the dorsal … Discussion Doses of estrogen and G-1 Doses of estradiol as well as routes of administration in female rodents vary widely (Lund et al. 2005), depending on the study’s endpoints. As we hypothesized that the chronic G-1 effect on cognition (Hammond et al.