Ersidade Paranaense. The experiments were carried out in the morning, and the Beleuchtungsst Strength was 200 lux in the experimental room. 2.5.1. The elevated plus maze increased Hte maze for Mice consisted of two perpendicular arms open and closed, the two perpendicular arms were open at the top. The maze was constructed of wood was painted black and was raised 45 cm above the ground. Open arms were surrounded by a wooden bar to M Mice from falling to prevent the labyrinth. One hour after oral treatment, the mouse in the middle of the erh Brought Hten maze facing a closed arm and observed for 5 min. W during the trial period, the following variables were measured: the number of entries and excursions in the time spent on open and closed arms Maraviroc UK-427857 and total arm scanned. Arm entries GE was defined as the input of all four paws in the arm. Anxiolytic compounds selectively increased Hen the percentage of time on open arms and percentage of open arms entries spent GE. These effects were not Changes in locomotor activity t, measured in increased Hten maze, observed that the total number of ballots arm. Before each test, the labyrinth with a L Solution of 10% ethanol and clean, dry cloth. 2.5.2. Open field test activity was t in the open field in a soft acrylic-K Fig with a black floor with S lines in the fields marked 10 cm2 measured. One hour after drug administration, each mouse was in the arena, and it going Ability placed in the peripheral zone, breeding, grooming, and defecation were recorded for 5 min. The number of intersections, which was min the grid of the two Hinterfü S over a period of 5 as an index of walking. After each test the apparatus in the open field has a L Purified solution of 10% ethanol. 2.5.3.
Marble burying test one hour after drug administration, each mouse was individually in a K Fig from propylene, which was identical to its K Cage and contained 5 cm deep south Sawdust and 24 glass beads Equidistant own against the wall. No food or water was available. The number of balls that at least two thirds were recorded buried after 30 min. 2.6. For the preparation of flunitrazepam binding membrane, were the brains of rats in a homogenization buffer. The homogenate was centrifuged at 4 centrifuged to 1000 g for 10 min and the supernatant was collected from this first centrifugation and centrifuged again at 4 to 16,000 g for 20 min. The pellet was resuspended in the same buffer and frozen at 0 for 48 hours. After this period, the Bcr-Abl inhibition membranes were thawed, washed and resuspended in 50 mM Tris-HCl and 2 mM EDTA and centrifuged at to 16,000 g for 10 min. The pellet was resuspended in the same buffer and incubated at 37 for 30 min. After incubation, the pellets were centrifuged and described above under the same conditions twice. After the last centrifugation the pellets were resuspended in 20 mM HEPES and 1 mM EDTA, and the protein content was determined according to Bradford. The binding assay was performed as previously described flunitrazepam. The incubation was in duplicate in R Hrchen containing 50 mM Tris-HCl polycarbonated, 0.5 mg of membrane protein in the absence or presence of the crude extract performed hydroéthanolique yarrow plant. Diazepam was used as controls Positive. The concentration of diazepam was based on the previo.