After incubation proteins were separated by SDS-PAGE electrophore

After incubation proteins were separated by SDS-PAGE electrophoresis and detected by Western blot hybridization with anti-LytM antibodies. (TIFF 129 KB) Additional file 3: Time course of S. aureus 8325–4 cell lysis by LytM185-316 and lysostaphin in various conditions. (A) Influence of glycine. Lysis experiments were done in 100 mM glycine-NaOH, pH 8.0, 50 mM Tris-HCl, pH 8.0 and 100 mM glycine in 50 mM Tris-HCl pH 8.0. (B) Influence of mono-, di- and triglycine. Buffers SB202190 clinical trial were made as 50 mM with pH adjusted to 8.0 with NaOH. For comparison lysis in dd water was also checked. (C) Influence of various aminoacids. 50 mM L-arginine-HCl, D,L-alanine-NaOH,

L-arginine-HCl, L-glutamic acid-NaOH, diaminopimelic acid (DAP)-NaOH of pH 8.0 were tested. Lysis experiments were performed as described in Material and Methods. (TIFF 877 KB) Additional file 4: Histological examination of mouse ear during the development of eczema and S. aureus infection. (A) section of control ear, (B) section 2 days after S. aureus infection; massive invasion of inflammatory cells can be observed (indicated with open arrows). (TIFF 2 MB) References 1. Jones RN, Ballow CH, Biedenbach DJ, Deinhart JA, Schentag JJ: Antimicrobial activity of quinupristin-dalfopristin

(RP 59500, Synercid) tested against over 28,000 recent clinical isolates from 200 medical centers in the United States and Canada. Diagn Microbiol Infect Dis 1998,31(3):437–451.PubMedCrossRef Ro 61-8048 in vivo 2. Brickner SJ, Barbachyn MR, Hutchinson DK, Manninen

PR: Linezolid (ZYVOX), the first member of a completely new class of antibacterial agents for treatment of serious gram-positive infections. J Med Chem 2008,51(7):1981–1990.PubMedCrossRef 3. Borysowski J, Weber-Dabrowska B, Gorski A: Bacteriophage endolysins as a novel class of antibacterial agents. Exp Biol Med (Maywood) 2006,231(4):366–377. 4. Trayer HR, Mdivi1 order Buckley CE: Molecular properties of lysostaphin, a bacteriolytic agent specific for Staphylococcus aureus. J Biol Chem 1970,245(18):4842–4846.PubMed 5. Mani N, Tobin P, Jayaswal RK: Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis. J Protein kinase N1 Bacteriol 1993,175(5):1493–1499.PubMed 6. Ramadurai L, Lockwood KJ, Nadakavukaren MJ, Jayaswal RK: Characterization of a chromosomally encoded glycylglycine endopeptidase of Staphylococcus aureus. Microbiology 1999,145(Pt 4):801–808.PubMedCrossRef 7. Recsei PA, Gruss AD, Novick RP: Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc Natl Acad Sci U S A 1987,84(5):1127–1131.PubMedCrossRef 8. Heinrich P, Rosenstein R, Bohmer M, Sonner P, Gotz F: The molecular organization of the lysostaphin gene and its sequences repeated in tandem. Mol Gen Genet 1987,209(3):563–569.PubMedCrossRef 9. Thumm G, Gotz F: Studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (Lif) of Staphylococcus simulans biovar staphylolyticus.

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