Angiogenesis is one of the key steps in tumor growth and metastasis. The tumor suppressor gene is another important factor in tumorigenesis. Vascular endothelial growth factor (VEGF)-D and hypoxia inducible factor (HIF)-1а are angiogenetic factors, and annexin A7 (ANXA7) exhibits biological and genetic properties expected of a tumor suppressor gene. This study was retrospectively analyzed 978 EGC patients who underwent curative resections at the Chungnam National Hospital between PXD101 purchase March 2005 and December 2009 in order to analyze VEGF-D, HIF-1а, and ANXA7 expression. Methods: Immunohistochemical staining revealed that the expression of VEGF-D was higher and the expression of ANXA7 was lower in the
LN metastasis group than in the non-LN metastasis group. HIF-1а, however,
did not stain in both groups; hence, we statistically evaluated VEGF-D and ANXA7 expression. VEGF-D and ANXA7 were significantly correlated with LN metastasis; however, EGC morphology, size, and histology were not found to be positively correlated with VEGF-D and ANXA7 expression. Results: Tumor size, depth of invasion, and histologic differentiation were associated with lymph node metastasis in EGC. This study also found a close relationship between LN metastasis and the expression of VEGF-D and ANXA7. Thus, LN RG7420 metastasis and the presence of lymphovascular tumor emboli should be considered in small EGC, especially flat depressed EGC. Conclusion: This study also found a close relationship between LN metastasis and the expression of VEGF-D and ANXA7. Key Word(s): 1. Early gastric cancer; 2. LN metastasis; 3. VEGF-D; 4. HIF-1а, ANXA7; Presenting Author: HONG-GANG YU Additional Authors: WEI ZHOU Corresponding Author: HONG-GANG YU Affiliations: Institute for Gastroenterology and Hepatology,
Renmin MCE Hospital of Wuhan University,; Department of Gastroenterology, Renmin Hospital of Wuhan Univeristy, Objective: To investigate expression and function of Notch-1 and PTEN in gastric cancer tissues and cells, study the regulatory mechanism of PTEN expression and explore the specific role in the resistance to doxorubicin in gastric cancer. Methods: 1. Forty four adult patients with gastric cancer were collected for the study. Observe the expression of Notch-1 and PTEN in gastric cancer tissues. 2. PTEN luciferase reporter gene construction was used to detected PTEN promoter activity under different conditions. 3. RNAi interference technology was uesd to downregulation Notch-1 expression, after transfection, the effects of Notch-1 downregulation on transcription factor CBF-1 activity in the presence or absence of doxorubicin were investigated by EMSA, on PTEN expression were investigated by Key Word(s): 1. Notch1; 2. PTEN; 3. chemoresistance; 4. gastric cancer; Presenting Author: SALIMM. A. BASTAKI Additional Authors: NAHEED AMIR, RASHEDS HAMEED, SAEED TARIQ, ERNEST ADEGHATE Corresponding Author: SALIMM. A.