1 and 2 mRNA and HIF reducing BRL-15572 the contraction IVC prevented with the end of the vein wall are associated with a compatible engaged Ngerten r The HIF-1 and 2 reduced the contractile response HIF w During vein wall route. It also supports the observation that HIF stabilizerDMOG not reduce HIF-1 and HIF-2 mRNA expression while reducing the contraction of the contention that HIF-1 and HIF 2 are connected with the reduced contraction in the end involved in expanding vein. We investigated whether the regulation of the curves Sen contraction by mechanical stretch and HIF includes MMP. Studies have shown that the expression and activity of t the regulated MMP-2 and MMP 9 were from HIF.39, 40 In line with our previous report, 6 the l Ngere erh Relationships in the IVC with an increased blood Hten Associated expression of MMP 9 and MMP-2 mRNA and protein. An important finding is that increased Hte MMP-2 and MMP was inversely 9 mRNA associated with the tip dilated vein through the HIF inhibitor U0126, 17 DMAG and echinomycin, our hypothesis that the overexpression of MMP 2 and MMP 9, rats to l-tron Ngere mechanical IVC subject It is regulated by HIF. Moreover, the VCI is in rats with DMOGduring L Ngere Erh Were treated relationships in the blood vessel wall, but showed up-regulation of MMP 2 and MMP 9 mRNA and a further reduction of the contraction IVC, in accordance with a potential between routes vein L Ngere mechanical, increases hte expression of HIF, the upregulation of MMP, and reduced curves sen contraction. The mechanism of regulation of HIF by mechanical strain is unclear, but may be PI3K and MAPK.14, 15.18 canals le can in cell membranes, integrins, ion and receptor tyrosine kinases are mechanosensitive to stretch stretch.41 mechanics at the activation of PI3K Ca2 influx through ion channels le passenger stimulate potential receptors such as the transient receptor potential cation channel subfamily V member and 4.42, k integrins can transduce mechanical strain to initiate.
MAPK signaling cascades and activation.43 receptor tyrosine kinases and G-protein-coupled receptors are also stimulated by improper loading, with subsequent final activation of MAPK.44 In addition, the mechanical strain erh can activate or inhibit the increase of reactive oxygen species MAPK.45, U0126 MAPK.15 46 18 The observation that the expression of HIF 1 and HIF-2 mRNA increased ht and reduces the duration of contraction with stretching IVC connected by U0126 schl reversed gt an r MAPK in the regulation of HIF by mechanical stretching. Although 17 DMAG inhibited the overexpression of HIF 1, HIF 2, MMP 2 and MMP 9, it has undergone no reversal of the reduced contraction in IVC, an L Ngere route. 17 DMAG is a protein derived from geldanamycin-heat shock protein 90 inhibitor, destabilization and degradation Silibinin of HIF f Promoted. Hsp90 stabilizes HIF, by acting as a molecular chaperone that employees with nuclear HIF w During translocation.8, 14 However, Hsp90 can affect the vascular and regulate other ways Tonus by nitric oxide synthase and superoxide anion.47, 48 The inhibition of 17-DMAG Hsp90 M possible effects are not related to inhibition of HIF venorelaxation. CONCLUSION l Ngere erh Associated relationships in the blood vessel wall with overexpression of HIF-1 and HIF 2, increases hte MMP 2 and MMP 9 expression, and reduced curves Se c.