Expressing mGFP alone with all the scalloped GAL4 driver won’t result in a phenotype , but expressing CagA induces apoptosis inside the wing blade area with the imaginal disc . Applying the apterous GAL4 driver to express mGFP alone will not induce a phenotype , but expression of CagA triggers apoptosis while in the dorsal wing blade region of the imaginal disc . Expressing mGFP alone with all the engrailed GAL4 driver will not induce a phenotype , but expressing CagA triggers disruption on the imaginal disc epithelium . Implementing the patched GAL4 driver to express mGFP alone won’t bring about a phenotype, but expression of CagA triggers slight epithelial disruption and pretty mild apoptosis inside the wing blade region within the imaginal disc . Scale bar, 50 mm. Adult wing pictures from male flies expressing mGFP and CagA together with the indicated GAL4 driver, which display various amounts of epithelial disruption. Scale bar, 500 mm.
Confocal cross sections of third instar larval wing imaginal discs exhibiting mGFP expression, and stained with an antibody towards lively caspase three to mark apoptotic describes it cells and phalloidin to reveal f actin construction. Expressing CagA with bx GAL4 disrupts ordinary epithelial architecture most appreciably in regions within the wing imaginal disc that are undergoing apoptosis . Epithelial disruption is a lot more substantial in wing imaginal discs expressing two copies of CagA with bx GAL4, which exhibit this phenotype all through the tissue . Scale bar, 50 mm. Confocal cross sections of male third instar larval wing imaginal discs exhibiting mGFP expression with bx GAL4 and stained with anti lively caspase 3 antibody to mark apoptotic cells.
Ectopic overexpression of p35 within the dorsal wing disc doesn’t bring about a phenotype , and coexpression with CagA suppresses the apoptosis generally caused by CagA expression . Scale bars, 50 mm. Adult wing photographs from male flies expressing the apoptosis inhibitor p35 alone or in combination with CagA. Ectopic expression of p35 with bx GAL4 will not lead to a phenotype , even though coexpression with the advantage CagA enhances epithelial disruption . Scale bar, 500 mm. Confocal cross section of the male wing imaginal disc epithelium carrying the puc lacZ reporter allele and expressing mGFP and CagA with bx GAL4. Staining with antibodies towards bgalactosidase and phosphorylated JNK demonstrates that puc lacZ upregulation correlates with JNK phosphorylation. Scale bar, 50 mm. Confocal cross sections of male third instar larval wing imaginal discs exhibiting mGFP expression with bx GAL4 and stained with anti lively caspase three antibody to mark apoptotic cells.
RNAi mediated knockdown of polarity determinants Baz , Crb or Par1 alone from the dorsal wing doesn’t induce apoptosis. Coexpression of CagA with knockdown of Baz , Crb or Par1 won’t boost the apoptosis phenotype.