In addition, multivariate regression analysis showed that 3 parameters (donor type, eGFR at 2004 and total or high-molecular

ADPN levels) were independently related to the initial DeGFR in renal transplanted subjects. Low-molecular weight adiponectin ratio was significantly increased at last 4 year (P < 0.001 R428 clinical trial by the paired t-test). The late 4 years DeGFR became slower than those of initial levels at −1.1(−8.2∼3.2) ml/min/1.73 m2/year in 85 subjects. The late DeGFR was related with the alteration of HDL-C or low-molecular ADPN levels (r = 0.317, p = 0.006; r = −0.260, p = 0.026, respectively). Conclusion: Lower LDL-C/HDL-C ratio and the usage of statin itself could preserve the renal function judged

by DeGFR in Japanese transplanted subjects. Initial ADPN levels were reversely correlated with eGFR and DeGFR, like previously reported as an “ADPN paradox” even in transplanted subjects. However, long-term observation revealed that higher HDL-C and lower low-molecular ADPN levels preserved the renal function of allografts, and resolved the paradox between the renal function and ADPN levels mainly caused by the increase of low-molecular ADPN in renal allograft dysfunction. SHIGA TAKAHIRO1, TANAKA HARUKA1, ISHIDA KAORI2, KAWATA TETSUNORI2, SUZUKI TSUKASA1, YAMAMOTO YUJI1, KOBAYASHI KEN-ICHI1 1Dept. Appl. Biol. Chem., Tokyo Univ. of Agri.; 2Grad. Sch. of Edu., Okayama Univ. Introduction: Vitamin B12 is a water soluble Rapamycin vitamin, serves as an essential cofactor for two enzymes, methionine synthase and metylmalonyl-CoA mutase. Vitamin A is a fat-soluble vitamin, plays a role in a various functions, such as vision, immune function, embryonic development, and gene transcription. A common reabsorption receptor of these vitamins in the kidney is megalin that is a 600 kDa type 1 transmembrane protein. However, mutual relationship between these vitamins in the megalin mediated reabsorption is not well understood. The aim of this study is to Myosin reveal the effect of vitamin B12 deficiency on renal reabsorption of

vitamin A. Methods: Wistar rats weaned from parent rats fed on a Vitamin B12 deficient diet during pregnancy and lactation were divided four groups, (1) Control; group administered 1 ug/rat/day of cyanocobalamin (CNB12) for 100 days, (2) B12-Def, (3) 24 hrs-CNB12; group administered 1 ug/rat/day of CNB12 for a day before sacrifice, and (4) 7days-CNB12; group administered CNB12 for 7 days before sacrifice. These rats were fed on Vitamin B12-deficient diet for 100 days. Serum Vitamin B12 and vitamin A were measured. Localization of megalin, cubilin and retinol-binding protein (RBP) was investigated by immunohistochemistry using light and laser confocal microscopy. The mRNA and protein expression of megalin and RBP were analized by real-time PCR and western blotting respectively.