In this test, older adults stand up from a sitting position in a chair as often as they can in 30 seconds. The chair-stand test has a reliability (test-retest) of r = 0.88 and a convergent validity of r = 0.75. To be included in the study, respondents to the study advertisement had to be over 55 years old and to experience regular episodes of nocturnal leg cramps, defined as at least once per week. Potential participants were excluded if they were using quinine or medication to assist sleep. They were also excluded if they had orthopaedic problems, severe medical conditions, or comorbidities known

to cause muscular spasms or cramps. Participants in the experimental group attended a 45-min visit at which they were taught a program SAHA HDAC supplier of daily stretching exercises for the hamstring and calf muscles by one physiotherapist, who was specially trained in the Crenolanib cost study procedures. Participants were advised to perform the stretches in standing, as presented in Figure 1a and b and described in Box 1. For each stretch, the participant was advised

to adopt the position shown, move to the comfortable limit of motion, move beyond this to until a moderately intense stretch was felt and sustained for 10 seconds, and then return to the starting position. Participants were instructed to remain calm and never to hold their breath during the stretch. Each stretch was performed a total of three times, with 10 seconds of relaxation between each stretch. Stretching of both legs was done within three minutes. The physiotherapist demonstrated the stretches first and then observed the participant performing the stretches, correcting the technique if necessary. If a participant found stretching in standing difficult, the participant was shown how to Cell press stretch in a sitting position, as presented in Figure 1c and

described in Box 1. Stretch Description Calf stretch in standing Starting position. Standing facing a wall with the elbows extended and both palms on the wall at chest height. One leg is forward with the knee flexed and the other leg is back with the knee extended. Both feet are in full contact with the floor. Motion to apply stretch. Flex the front knee so that the trunk moves forward, keeping the trunk straight and the heels in contact with the floor. Hamstring stretch in standing Starting position. Standing facing a chair that is placed against a wall. Place one heel on the chair with the knee of that leg fully extended. Motion to apply stretch. Flex at the hips so that the trunk tilts forward, keeping the trunk straight. The foot on the floor should maintain full contact and the other heel remains in contact with the chair. Hamstring and calf stretch in sitting Starting position. Sit on the floor or a firm bed with both legs extended. Grasp toes with both hands. Motion to apply stretch.