It was down regulated at both three weeks and towards the end of regeneration, sug gesting a role in preventing a order inhibitor continued vascularization process 4 The lack of TGF B gene expression and ELISA confirms the findings from Oe et. Inhibitors,Modulators,Libraries al, verify ing the assumption that intact signalling by TGF B is not required for termination of liver regeneration. Methods Experimental setup Twelve female Norwegian landrace pigs, weighing 31. 7 kg from a single commercial farm were used. The animals were housed in a closed system indoor fa cility with 55 10% relative humidity, 17 18 air changes per hour and temperature of 20 1 C. The pigs shared fenceline contact with Inhibitors,Modulators,Libraries another related pig and were sin gly housed in 1. 5 1. 5 m pens with ad libitum access to tap water from water nipples, liquid dietary supplement and digestive energy mixed with water.
Light was sup plied on a 12 12 hour schedule. Four pigs were subject to a 60% PHx, four pigs were subject to sham surgery and four pigs were used as controls. Control ani mals were necessary, as all of these animals were grow ing, and Inhibitors,Modulators,Libraries a measurement of normal liver growth was needed. All pigs were re operated at three and at six weeks post PHx. Biopsies were sampled upon initial laparotomy, at three weeks post PHx and upon termination at six weeks post PHx. This project was approved in agreement with the Norwegian Animal Welfare Act 21 and The Norwegian Regulation on Animal Experimentation 7, 8 and 13. Our department is run in agreement with the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes.
Anaesthesia The animals were fasted overnight with free access to water. They were initially sedated with Ketamin and Atropin. All animals were intubated, and anaesthesia was maintained with Isoflurane 1. 5 2% mixed with 50 60% oxygen. Respiratory rate was adjusted to achieve an Et CO2 be tween 35 and 40 mmHg. Intravenous access Inhibitors,Modulators,Libraries was obtained through a vein on the ear. Analgesia was induced and maintained with Fentanyl 0. 01 mgkg, i. v. All animals received a peroperative i. v. volume Inhibitors,Modulators,Libraries load con sisting of 1000 ml http://www.selleckchem.com/products/Belinostat.html Ringer solution. Volume infusion was continued thereafter with 20 mlkghr 0. 9% NaCl and 10% Glucose. Before surgery, all animals received a single intramuscular injection of antibiotic prophylaxis with Enrofloxacin 2. 5 mgkg.