It was later validated as
a broad measure of abnormal eating patterns and is now used as a screening tool for undifferentiated eating disorders in high-risk populations [24, 25]. Presently, the EAT-40 is considered the most widely used self-report measure of disordered eating  and has been used in prior studies with elite skaters [14, 17]. The EAT-40 has a high degree of internal reliability with Cronbach’s alphas ranging from 0.79-0.94 ; measures greater than 0.7 are acceptable . The EAT-40 is a self-reported 40-item instrument answered on a 6-point Likert-type scale (1 = never, 6 = always). The instrument is scored by assigning points to each response (3 points for the most “symptomatic” response, 2 points for Temsirolimus purchase the next “symptomatic” response, 1 point for the least “symptomatic” response, and
no points for “non-symptomatic” responses) and summing scores for all 40 items . EAT-40 scores >30 this website indicate the presence of clinically significant eating pathology [24, 25]. Physical activity level Three 24-hour records of physical activity were collected on the same three days participants recorded their dietary intakes to estimate physical activity level during a period of active training. Participants reviewed the activity records with a study staff member during the first week of training camp to clarify missing or ambiguous data, and means were calculated. Blood chemistries A 12-hour fasting blood sample (25 ml) was obtained
by STI571 order venipuncture from each skater on the first morning after arrival at the training camp and analyzed triclocarban for hematologic indices (serum iron, total iron binding capacity, total iron saturation, serum ferritin, hemoglobin and hematocrit) and serum albumin (Pikes Peak Diagnostic Service, Inc., Colorado Springs, CO). Data analysis All data were analyzed using the SPSS for Windows statistical program (version 7.0, 1997, SPSS, Inc., Cary, NC). Means and standard deviations were calculated for each variable to provide descriptive information on the anthropometrics, nutrient intake, EAT-40 scores and biochemical indices of nutritional status for the skaters. Results Table 1 describes the characteristics of these competitive adolescent female figure skaters. The 36 participants ranged in age from 13–22 years with a mean and median age of 16 years. The group had a mean BMI of 19.8 ± 2.1 SD (median 19.9) with a range from 15.1 – 23.3. All skaters >19y had normal BMIs compared to adult standards. All but one of the skaters ≤19y had a BMI-for-age within the healthy weight range (5th to 85th percentile) using age- and gender-specific CDC growth charts . Based on these charts, 1 skater had a BMI-for-age <5th percentile and would be classified as “underweight,” 7 skaters were between the 5th-25th percentile, 13 skaters were between the 25th-50th percentile, 9 skaters were between the 50th-75th percentile and 2 skaters were between the 75th-85th percentile.