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the manuscript. CZZ led the experiment and supervised the project. MW prepared the samples and performed the characterization. ST and PC participated in the discussions. PK completed the measurement. All of the authors read and approved the final manuscript.”
“Background Hydrophilic tips used in scanning near field optical microscope (SNOM) condense some water layers, leading to the formation of a water bridge (or water meniscus) between the tip

and a hydrophilic sample for small tip-sample distances. The shape of such meniscus will depend on the geometry of both surfaces, their separation, and environmental conditions (temperature and relative humidity). When working in air conditions using local probes, Phosphoribosylglycinamide formyltransferase humidity causes characteristic jump-to-contact events due to the spontaneous formation of a water meniscus between tip and sample [1]. Presence of water at experimental relatively high humidity conditions also modifies the dielectric properties of the medium between the SNOM tip and substrate. As a consequence, the optical images of samples on surfaces are altered by humidity and water condensation. Previous studies on the optical signal under variable environmental humidity [2, 3] have shown the conditional increase in the optical signal depending on the hydrophobic character of the sample. In fact, the inclusion of water condensation should be considered for any modeling or simulation of the field enhancement effect [3].