Our group has already proved that both Curcuma Wenyujin and its extracts show great effects

in anti-inflammation and anti-cancer. Methods: Taking SGC7901 as the negative control group, we use MTT to prove Whether SGC7901/VCR is a kind of multidrug resistant cell lines and draw a Volasertib solubility dmso growth curve of SGC7901 and SGC7901/VCR cultivated without VCR, and to choose non-toxic dose of Curcuma Wenyujin ethanol extract (CWEE). Then to prove whether non-toxic dose of Wenyujin can reverse MDR by MTT. Testing CD44 of both SGC7901 and SGC7901/VCR by flow cytometry to see whether it is a mark of cancer stem cell. We also use flow cytometry to test the effect of CWEE on apoptosis rate induced by VCR and cycle arrested by VCR. Through Western blot, we can see if CWEE can regulate the expression of Pgp and LRP. Then we further test the location of Pgp by IHC. To get check details a clear understanding of how CWEE affects the expression of Pgp and MRP1, we use RT-PCR to test the mRNA of Pgp and MRP1. Results: This study has proved that the SGC7901/VCR is a kind of multidrug resistant cell lines which resists Vincristine (VCR), Adriamycin (ADR), 5-fluorouracil (5-FU) and cis-platinum

(DDP). Among these chemotherapeutics, the cell line has a strongest resistance (5259.22 ± 358.08-fold) to the VCR while it has a least resistance (1.37 ± 0.16-fold) to DDP. When it is cultured without VCR, it proliferates just like the nondrug resistant cell line SGC7901 in the first week, but the former proliferates much MCE more quickly in the second week. flow cytometry shows there is no difference of CD44

between SGC7901/VCR and SGC7901. MTT and flow cytometry reveal that CWEE can reverse the resistance of SGC7901/VCR to VCR, ADR and 5-FU which depends on the concentration of CWEE. Flow cytometry shows that CWEE can enhance apoptosis rate of SGC7901/VCR induced by VCR and increase the ratio of cells in G2/M stage arrested by VCR. Both Western blot and IHC show that Pgp and LRP expresses much higher in SGC7901/VCR than in SGVC7901. However, only Pgp can be reduced by WEE. The interesting thing is that RT-PCR reveals CWEE increases the transcription of Pgp. Both Western blot and IHC show that Pgp and LRP expresses much higher inSGC7901/VCR than in SGVC7901. However, only Pgp can be reduced by CWEE. RT-PCR also shows that CWEE can reduce the transcription of MRP1. Conclusion: SGC7901/vcr is a good cell line of MDR for experiments. SGC7901/VCR is more aggressive than SGC7901. CD44 may have no relation with SGC7901/VCR’s drug resistance. To be more exact, CD44 may not be considered as an independent mark of cancer stem cell. we may infer that CWEE reverse MDR mainly by inhibiting the process of translation instead of transcription of Pgp as well as the transcription of MRP1.