Ment with a different pattern of uptake, intracellular storage and distribution Ren, And the result of biological effects in drug-free, PazPC micelle and the complex ion pair PazPC and drugs. So far it is unclear how P-glycoprotein the micelles PazPC drug absorption and cytotoxicity t Leuk to improve Chemistry cells. However, oxidized phospholipids, a type k Rpereigene substances which have a variety of biological activity Th sentieren to pr. PazPC can interact with mitochondria and lead to cytochrome c and apoptosis-inducing factor release from mitochondria in the cytoplasm and nucleus respectively. The mitochondria can k Not only coated Interred start apoptosis cascade, but also suppress the production of ATP. The first effect is probably t Th tumor cells in synergy with anti-tumor drugs, and it can affect the function of proteins associated ATP-dependent Independent MDR, such as P-glycoprotein, which then causes no increased Hte intracellular re drug. In addition, some OxPLs, for example, OxPAPC increased hen Can intracellularly Ren levels of reactive oxygen species and cancer cells are anf Llig of oxidative stress induced by the addition of exogenous ROS-generating agents. Erh hte ROS in cells and P388 P388/ADR of PazPC was in our laboratory. Probably k Nnte the increase in ROS induced in PazPC leuk Mix cells is another mechanism for the T Maintenance of cancer cells in synergy with the drug to be. Mechanisms to increased retail PazPC micelles Hten uptake and cytotoxicity t in leuk of DOX and IDA Mix induce cells to be studied in our laboratory. 5th Conclusion We have thought U and has developed a novel micellar formulation, built by an oxidized phospholipids PazPC only for the delivery of cancer drugs, anthracycline DOX and IDA. The complex ion pairs between PazPC DOX and tats Chlich is formed and then End the drug-loaded micelles were a 5:1 molar ratio PazPC Manufactured ratio of lipid to the drug, with a high encapsulation efficiency, low particle E and a good stability t under physiological conditions.
The drug-loaded micelles PazPC sensitive to pH Changes with accelerated release of DOX or IDA in an acidic environment. More importantly, it improves the absorption of the drug and showed a gr Ere effect on both the cytotoxicity t susceptible and resistant Leuk Preconcentrated, purified in comparison with the drug. Therefore, the micelles oxidized phospholipids based system k Nnte a promising medium for the anthracycline anticancer drugs t Be TIG and improve the treatment results of leukemia Chemistry. Acknowledgments We thank Professor Daniel Parsons for critical reading. This study was supported by the authors to the Institute. Cancer treatment of oxidative stress, treatment with xenobiotics, a Erh Increase the intracellular cause Ren reactive oxygen species. ROS are natural by-products of the mitochondrial metabolism and cells maintain a low level of ROS, because they have a r In the cellular Re signaling pathways important. However, h Higher levels, k ROS can directly Sch Hedgehog Pathwy The cause of the DNA, induce lipid peroxidation, membrane, which signals to the destabilization and initiate apoptosis. We showed that D-penicillamine, an aminothiol, dose- Independent generated ROS in cancer cells and is cytotoxic. Recently, the involvement of the p53 tumor suppressor protein that is highly sensitive to cellular Cellular level of ROS in apoptotic cell death induced by the pin involved D. However, a thiol-reactive.