Reduction of serum albumin in paracetamol treated group

may be due to formation of protein adduct. Catalase an enzymatic antioxidant protects the tissues from highly reactive hydroxyl radicals by converting the harmful hydrogen peroxide into water and oxygen.25 The reduction in the activity of this enzyme may induce oxidative stress in cells as a result of accumulation of toxic metabolites/radicals like superoxide radicals and hydrogen peroxide due to administration of PCM.26 Increased activity of catalase in animal’s co-administration with MEMV shows the preventive role of MEMV related to the accumulation of excessive free radicals in liver and thereby protecting the liver from paracetamol intoxication. The elevated level of MDA, the end products of lipid peroxidation in the liver tissue is important indicators of tissue PI3K inhibitor damage and failure of antioxidant defense mechanisms to prevent the formation of excessive free radicals in paracetamol intoxicated animals.27 The significant decline in the concentration of these constituents in the

liver tissue of PCM + MEMV and standard administered rats indicates anti-lipid peroxidative effects. GSH, the major non-protein thiol in living organisms removes free radical species such as hydrogen peroxide, superoxide radicals and maintains membrane protein thiols depleted in hepatic mitochondria during hepatic injury due to toxins. The GSH levels were significantly depleted in paracetamol treated group which due its conjugation with NAPQI to form mercapturic acid.28 The increased levels of glutathione in groups treated with MEMV reveal its ability to reduce oxidative stress. Our studies showed

UMI-77 price that the treatment of animals with MEMV significantly restored the metabolic enzyme activities at all doses which indicate they improved the physiological functions in liver tissue. This is also supported by the regulation of triglyceride levels. Histopathological studies also provided supportive evidence for biochemical analysis. MEMV treatment significantly improved cellular morphology in dose dependent manner. These results suggest that the hepatoprotective action of MEMV might be due to the presence of antioxidants isothipendyl (phenolic type (87%) or flavonoidal type) i.e. marrubiin, marrubinol and monoterpene like marrubic acid present in M. vulgare 29 which have proven antioxidant activity. 200 mg/kg of MEMV showed more effect than 100 mg/kg and was also equivalent to the standard as shown by the percent protection indicating improved cellular stability and metabolic activity. In conclusion the study revealed the hepatoprotective effect of the M. vulgare (200 mg/kg) against paracetamol induced injury. Further studies need to be carried out to fully characterize the mechanism responsible for antioxidant activity present in the extract and elucidate its possible mode of action and that is in progress. All authors have none to declare. “
“Hepatocellular carcinoma (HCC) is an aggressive tumor.