Statistical analysis results showed that LCMR1 expression was significantly associated with clinical stage of these NSCLC patients (P < 0.05), but no significant association was found between LCMR1 expression and other clinicopathologic parameters such
as gender, age, smoking status, pathological type, and histologic grade (Table 2). We further used the stepwise forward logistic regression analysis to assess the effects of clinical stages on LCMR1 expression. Logistic regression analysis revealed that an increased clinical stage was significantly associated with high LCMR1 expression (OR = 3.410, P = 0.026) (Table 3). The expression of LCMR1 protein in metastatic lymph nodes had no relationship with the clinic features of NSCLC patients Batimastat in vivo (data not shown). Table 2 Correlations between LCMR1 expression and clinicopathologic characteristics of human NSCLC. n LCMR1 expression P Negative Positive Gender
Male 61 12 49 0.147 Female 23 8 15 Age(y) ≥65 22 4 18 0.471 <65 62 16 46 selleck chemicals Smoking status Yes 45 10 35 0.714 No 39 10 29 Pathological type Adenocarcinoma 41 10 31 0.614 Squamous cell carcinoma 40 10 30 Adenosquamous carcinoma 3 0 3 Histologic grade PD 28 6 22 0.918 MD 45 11 34 WD 11 3 8 Lymph node metastasis Yes 62 12 50 0.108 No 22 8 14 Clinical stage I-II 40 14 26 0.022 III-IV 44 6 38 Abbreviations: WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated. Table 3 Logistic regression analysis. Wald χ2 P OR TNM stage 6.995 0.026 3.410 Survival analysis Kaplan-Meier analysis of 65 cases of this group, with a median follow-up
of 31 months, showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension (Figure 4). But no statistical significance was observed in overall survival (OS) and progression-free survival (PFS) of these NSCLC patients using univariate survival analysis and multivariate survival analysis and COX proportional hazard model analysis (data not shown). Figure 4 Kaplan-Meier analysis of 65 cases follow-up. Carnitine palmitoyltransferase II The survival curve showed increased difference in survival rates between patients with high-level LCMR1 protein expression and patients with low-level LCMR1 expression, with overall survival time extension. Discussion Tumor development is a complex and Selleck GDC0068 multistage process involving many genetic alterations. It is essential to explore the molecular mechanisms of tumor formation and progression to develop rational approaches to the diagnosis and therapy of cancer, therefore, identifying dysregulated genes and proteins in neoplasms are critical.