The effluent was analysed by APHA, 1981.3 The fresh material of plant was collected from both sites non-polluted (ALTT Centre) and polluted (cycles manufacturing unit) area of Ghaziabad, UP, India. For colour reaction test Cromwell, 19554 & Trease and Evans, 19835 were followed. TLC was done According to the WHO, Geneva, 1998.6 Chlorophyll a, b and total chlorophyll (a + b) were determined according to Arnon, 1949.7 The effluent was analysed and the results are given in Table 1. The result shows the presence of alkaloids, saponin, tannin, lignin, protein, carbohydrate, suberin, glucoside, oil, sugars, steroids and absence of flavanoids in both the cases. Degree of change in colour reaction tests are
tabulated in Table 2. From the observation of TLC, it is found that the number of spots were higher in non-polluted plants than the polluted plants (Plate 1). The RF values are tabulated in Table 3. Chlorophyll a, chlorophyll b and Autophagy inhibitor total chlorophyll were observed 76.98%, 86.29% and 80.10% of control leaves samples (Plate 2). The results are tabulated in Table 4. The effluent samples collected from the industry selected for this study was
analysed for different physico-chemical parameters which showed higher values as compared to the standard values recommended by the Indian Standard Institute (I.S.I.; 1974, 1974 and 1977). Similar results were also obtained by Kumar, et al,1988.8 A critical observation on the data studied clearly indicate that plants growing at polluted sites were badly affected and there were a significant reduction R428 ic50 in number of parameters studied as compared to the plants growing at the control sites. Major qualitative changes, noticed under the impact of industrial effluent, are reduction in chlorophyll level, photosynthesis rate, accumulation of heavy metals, alternation in pH, BOD, COD, Colour, Temp, Odour, TS, TDS. Heavy metals resulted into reduced growth and yield in comparison to plant species growing at non-polluted sites. The impact of industrial effluent on the qualitative and quantitative
values of medicinal plants does not appear to have been undertaken much till now. Colour reaction tests showed the degree of changes in plants of polluted sites. From the observations some alteration in the bio-chemical parameters were also recorded in plants growing nearly near the industrial effluent. The amount of chemical constituents found to have decreased in those plants which were growing in polluted areas. From the observations of TLC, it was seen that the number of spots were decreased in the plant samples of polluted sites. From the findings of this investigation it may be ascertained that there had been qualitative and quantitative alternations in the chemical constituents in the plants growing in industrial areas. It can also be stated that industrial pollution may also have lowered the drug potency of the plants growing in the vicinity of industries.