The FAD2-4 coding region was expressed in yeast and shown to encode a functional delta-12 desaturase, converting oleic acid (C18:1) to linoleic acid (C18:2) in recombinant yeast cells. In addition, both the FAD2-4 and the FAD2-3 genes were transferred into the Arabidopsis see more thaliana fad2-1 mutant background where they effectively restored wild type fatty acid composition and growth characteristics. Finally, the cotton FAD2-4 green fluorescent protein (GFP) fusion polypeptide

appeared to be localized in the endomembrane system of transgenic Arabidopsis plants in the complemented fad2-1 mutant background, supporting a functional ER location for the cotton FAD2-4 polypeptide in this heterologous plant system. Thus, a new functional member of the FAD2 gene family in

cotton has been characterized, indicating a complex regulation of membrane lipid desaturation in this important fiber/oilseed crop. (C) 2009 Elsevier Masson SAS. All rights Selleck Vorinostat reserved.”
“About 5-10 % of chronic myelogenous leukemia (CML) patients show variant Philadelphia (Ph) translocations. The formation mechanisms and clinical significance of variant Ph translocations remain unclear. We report a CML case with a novel five-way complex translocation. Although the result of initial G-banding was 46,XY,t(7;11;9)(q22;q13;q34),t(9;22)(q34;q11.2), fluorescence in situ hybridization (FISH) demonstrated t(7;11;9;22;9)(q22;q13;q34;q11.2;q34) consisting of sequential rearrangements involving five

chromosomes. The patient was successfully treated by imatinib AZD7762 and obtained a major molecular response. To our knowledge, this is the tenth CML case with a complicated Ph translocation involving five chromosomes and the third one treated by imatinib. Good response with imatinib therapy suggested that a single-event rearrangement was involved in the chromosomal changes.”
“A poroelastic medium, comprising a deformable solid with a saturating fluid, exhibits pressure-driven fluid transport that, via a solid/fluid coupling, generates stresses in the surrounding solid. In an elastically anisotropic solid, this coupling may lead to interactions between distant fluid elements and, therefore, to complex transport behavior. To examine the impact of elastic anisotropy on poroelastic transport, a simplified phase-field model of fluid imbibition in a weakly anisotropic poroelastic solid is studied. It is found that the rate of imbibition depends on the strength of the crystalline anisotropy as well as the orientation of the crystallographic axes relative to the direction of a propagating fluid front. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3204649]“
“In marine bacteria and some thraustochytrids (marine stramenopiles) long-chain polyunsaturated fatty acids (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are produced de novo by PUFA synthases.