The most important difference was the positive influence for survival of north sides of retention trees on clearcuts which had become evident after 14 years, indicating that the advantage of this microhabitat increases with time. Another difference was a significantly

lower vitality for spring transplants than autumn transplants two years after transplantation but not after 14 years. Spring was unusually dry in the transplantation year, which evidently had a strong negative effect on the vitality of transplants mounted that season. But, the initial climatic BGB324 concentration differences may have been evened out during the following 12 years. In the current study we used generalized linear mixed models as a statistical tool in order to explain survival and vitality in L. pulmonaria transplants. Bortezomib This approach was chosen since we wanted to account for the random effects of study sites and trees, and hence to avoid pseudoreplication. Our results on transplant survival and vitality after two years differed slightly from the data analysis presented in Hazell and Gustafsson (1999), since they used ordinal logistic regression and a different set of explanatory variables (that were not measured in 2008). One example is the significantly higher vitality on the south side of forest aspens demonstrated

for the 1996 data with logistic regression of Hazell and Gustafsson but not with the GLMM used by us. Another is the significantly lower vitality shown for lichen transplants on scattered trees compared with trees in groups on clearcuts after two years using logistic regression, but not detected when using the GLMM on the same data. However, we believe that the GLMMs used here are more reliable since random factors were accounted for. Our study shows that retention of aspens at clearcutting can be of importance to the lichen L. pulmonaria,

and most likely also to other lichen species with similar habitat demands in the boreal zone. If not all aspens can be retained, such with L. pulmonaria should be prioritized, because it is an uncommon, Adenosine triphosphate red-listed species, and highest priority should be given to trees where it occurs on the north side. There are signs of continued decline in L. pulmonaria in Sweden ( Fritz, 2011) and if it reaches very low population levels, one alternative could be translocation of the species to new sites. Our study indicates that in order for this to be efficient, northern sides of trees are preferable, and a careful selection of transplantation occasion in periods of high precipitation and humidity is advantageous. Maintaining and also increasing the amount of old aspens and also other host tree species in heterogeneous forest landscapes will be a prerequisite for continued survival of L. pulmonaria in boreal N. Europe.