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“The present paper undertakes the study of the essential oils (EO) composition and trichomes distribution, morphology and anatomy of the aerial organs of Pelargonium graveolens L’Her. which is an aromatic and medicinal species originating from Sfax (Tunisia). In fact, three morphologically distinct types of trichomes which are non-glandular trichomes, capitates glandular trichomes and peltate ones were observed with scanning electron microscopy (SEM) and light microscopy (LM). Besides, the essential oils (EO) were extracted by hydrodistillation and their compositions were determined by DAPT nmr Gas Chromatography-mass spectrometry

analysis (GC-MS). While in flowers and leaves beta-citronellol was the dominant component of their EOs with 24.21% and 19.2%, respectively, in stems the EO was mainly composed of delta-selinene (14.21%). (C) 2013 Elsevier B.V. All rights reserved.”
“MAb1, a human IgG1 monoclonal antibody produced in a NS0 cell line, exhibits charge heterogeneity because of the presence of variants formed by processes such as N-terminal glutamate cyclization, C-terminal lysine truncation, deamidation, aspartate isomerization and sialylation in the carbohydrate moiety. Four major charge variants of MAb1 were isolated and the conformations of these charge variants were studied using hydrogen/deuterium exchange mass spectrometry, including the H/D exchange time course (HX-MS) and the stability of unpurified proteins from rates of

H/D exchange (SUPREX) techniques. HX-MS was used to evaluate the conformation and solution dynamics of MAb1 charge variants by measuring their deuterium Go 6983 chemical structure buildup over time at the peptide level. The SUPREX technique evaluated the unfolding profile and relative stability of the charge variants by measuring the exchange properties of globally protected amide protons in the presence of a chemical denaturant. The H/D exchange profiles from both techniques were compared among the four charge variants of MAb1. The two techniques together offered extensive understanding about the local

and subglobal/global unfolding of the charge variants of MAb1. Our results demonstrated that all four charge variants of MAb1 were not significantly different in conformation, solution NVP-BSK805 dynamics and chemical denaturant-induced unfolding profile and stability, which aids in understanding the biofunctions of the molecules. The analytical strategy used for conformational characterization may also be applicable to comparability studies done for antibody therapeutics.”
“Synovial sarcomas are malignant tumors of mesenchymal origin, extremely rarely located in the area of the head and neck. Histologically they can be monophasic, biphasic or poorly differentiated with numerous differential diagnostic dilemmas. A 54-year-old male with synovial sarcoma of the carotid space is presented. The patient refused suggested postoperative radiotherapy and, nine months after the primary surgery, local relapse was verified.