Therefore, oil pollution may change the species composition by selectively eliminating the dominant grazers among plankton which may lead to the increasing abundance of primary producers (Miller et al., 1978) and intensified eutrophication process. The current study focused mostly on direct damage and short-term effects of high

IDH inhibitor clinical trial (400–1700 mg/L) and low (10–100 mg/L) concentration crude oil on plankton survival. The used low concentrations are realistic of oil spill conditions (Bobra et al., 1989) whereas high concentrations are realistic in the case of emulsification process (Xie et al., 2007). The observed effects of high concentrations are plausibly due to the direct impact of oil on zooplankton, e.g. through inhibiting effect on glutamic oxalacetic transaminase activity (Biesinger and Christensen, 1972), gas-exchange inhibition (Pezeshki et al., 2000), and also direct feeding and

absorption of oil and its residues by the organism (Duesterloh et al., 2002). Besides, the chemoreception used by zooplankton during foraging and mating may be also misled by crude oil soluble fraction (Herbert and Poulet, 1980). More importantly, crude oil has been also proven to have influence on live tissues, cells, and genetic material (Bhattacharjee and Fernando, 2008, Carls et al., 1999 and Parab et al., 2008) which may interrupt the operation of physiological and biochemical system (Wezel and Opperhuizen, 1995) this website to the level that the photooxidation process can even take place at low concentrations (Karydis, 1982). In our study we observed that crude oil destructively influenced the

somatic structure of cladocerans, sometimes removing the whole carapace of the animal. This is likely due to the damaging effect on the parts standing for connecting the carapace to residual body. The survival rate was also PtdIns(3,4)P2 influenced by the insoluble surface layer of crude oil which immobilized some of the specimens that had moved up to surface, unable to move their appendages (Fig. 5). To date, most of the laboratory studies have focused on the water soluble components of crude oil (Bhattacharjee and Fernando, 2008, Duesterloh et al., 2002 and Martinez-Jeronimo et al., 2005). Focused research on the insoluble layer of crude oil would allow more thorough and generic conclusions about the oil pollution effects. Nevertheless, we believe that the water-soluble components may still be the key-factors to the cladocerans’ survival. Likewise in our experiment the impact primarily increased with raising oil concentration regardless of the insoluble layer of oil present at every concentration tested. Our experiment also demonstrated that all D. magna, which came into a contact with crude oil at concentrations below 100 mg L−1 had a promising recovery. However, above this threshold value, all cladocerans died a few days after transferring to clean water.