Worldwide, there is a need for continued wetland inventories as small water bodies have often been underemphasized, and many inventories are therefore unreliable [15, 22]. The use of remote sensing in fundamental temporary and permanent wetland ecology, moreover, is currently not widespread, but has a large potential. Some biologists claim that spatial scales of remote sensing and scales usually covered by ecological or evolutionary research do not match, thus creating a perception problem [8], limiting the use of remote sensing techniques in biological studies.The aim of this study is to http://www.selleckchem.com/products/AP24534.html indicate that elementary and relatively cheap imagery and basic remote sensing techniques can substantially improve the knowledge on characteristics of temporary and permanent wetlands. In this study isolated open water wetlands in the Cape region of the Western Cape were characterised from seven Landsat images using supervised classification methods. Classification results of the Landsat imagery were compared with those of an Envisat image. Ecologically relevant traits (surface area, distance, dynamics, total number, and fraction of temporary and permanent wetlands) were investigated and discussed within the scope of wetland ecology. The effectiveness as well as the limitations of this straightforward remote sensing study, as an addition to ecological research, were evaluated.2.?Materials and Methods2.1. Optical wetland detectionIsolated open water wetlands were classified from seven Landsat TM and ETM+ images acquired on 9 January 1987 (summer), 16 October 1990 (winter), 3 June 1999 (winter), 4 December 1999 (summer), 31 July 2000 (winter), 24 February 2001 (summer), and 3 June 2002 (winter). Images were downloaded from the Global Land Cover Facility (GLCF) website or purchased from the United States Geological Survey Organization (USGS). The study area is located in the Cape Region of the Western Cape Province, South Africa (within latitude 33�� 03�� to 33�� 52�� South and longitude 17�� 57�� to 19�� 05�� East). It has a Mediterranean climate, receives much of its rainfall in winter months, and has relatively dry summers [23].Ground truth data were collected for the larger (from 0.32 hectare onwards) wetlands in the area by field surveys in 2004 and 2005 and supplemented with information from topographical maps obtained from the South African Chief Directorate of Surveys and Mapping. Most of the vegetation of these larger water bodies was situated at the edges (personal observation). Band 4, which showed a strong contrast between water bodies and other land features, was used to define at least 25 training sites for each land cover type (fresh water, sea, mountains, two types of vegetation, city, and sand dunes).