Untreated CBA recipients and recipients given 0.1 g/kg of EPA rejected C57BL/10 hearts (median survival time [MST], 8 and 13 days, respectively). With a 1.0 g/kg dose of EPA, graft survival was markedly prolonged (MST > 100 days). To determine whether regulatory cells were generated, naive mice (secondary recipients) underwent adoptive transfer of splenocytes from EPA-treated primary recipients and cardiac allograft transplantation. Adoptive transfer of whole, CD4(+) and CD4(+)CD25(+)
splenocytes GSI-IX in vivo from EPA-treated recipients induced indefinite survival in secondary recipients. Flow cytometry showed that the CD4(+)CD25(+) cells were Foxp3(+). In reverse transcriptase-polymerase chain reaction (RT-PCR) studies, the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) mRNA was upregulated by EPA treatment. A PPAR gamma antagonist
abrogated the prolongation of graft survival induced by EPA treatment (MST, 13 days). Thus, in our model, purified EPA induced prolonged survival of fully mismatched cardiac allografts and generated regulatory T cells dependent on PPAR gamma activation.”
“BackgroundThe clinical diagnosis of fistulous tracts and recurrent fluid collections in hidradenitis suppurativa (HS) may be complex. Information on subclinical involvement and grading of severity may improve management.
ObjectiveTo study HS lesions and evaluate the relevance of adding ultrasound (US) to the clinical examination.
Methods and MaterialsWe STA-9090 reviewed the sonograms of consecutive patients with HS from January 1, 2010 to May 31, 2012. The abnormalities observed in the US examinations were organized, classified, and integrated into a clinical-sonographic scoring system (SOS-HS), to stage the disease.
ResultsThirty-four patients with HS with a total of 142 lesional areas were evaluated. US findings included subclinical
fluid collections in 76.4% of the AZD1480 ic50 patients, fistulous tracts in 29.4%, dermal pseudocysts in 70.6%, and widening of the hair follicles in 100%. Concordance with the clinical HS scoring performed by dermatologistsshowed a significant fair agreement (k=0.30; p<.001); concordance of SOS-HS with clinical scores was acceptable but significantly lower (k=0.27; p=.02) because clinical scores consistently underestimated disease stage and severity.
ConclusionUS examination of HS lesions provides anatomic information that is clinically unavailable. HS is possible to stage sonographically.”
“The role of polyamines (PAs) in plant reproduction, especially pollen development and germination has been demonstrated in several higher plants. The aim of the present research was to investigate PA involvement in pollen development and germination in dioecious kiwifruit (Actinidia deliciosa).