radiovictrix cultures. This work was performed under the auspices of the US Department of Energy Office http://www.selleckchem.com/products/kpt-330.html of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396, UT-Battelle and Oak Ridge National Laboratory under contract DE-AC05-00OR22725, as well as German Research Foundation (DFG) INST 599/1-2.
A representative genomic 16S rRNA sequence of strain Yu37-1T was compared using BLAST under default settings (e.g.
, considering only only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database  and the relative frequencies of taxa and keywords (reduced to their stem ) were determined, weighted by BLAST scores. The most frequently occurring genera were Deferribacter (33.4%), Alteromonas (21.3%), Magnetococcus (9.4%), Shuttleworthia (7.5%) and Geovibrio (7.3%) (61 hits in total). Regarding the single hit to sequences from members of the species, the average identity within HSPs was 99.9%, whereas the average coverage by HSPs was 96.7%. Among all other species, the one yielding the highest score was Deferribacter desulfuricans, which corresponded to an identity of 88.1% and an HSP coverage of 86.0%.
The highest-scoring environmental sequence was “type”:”entrez-nucleotide”,”attrs”:”text”:”DQ424925″,”term_id”:”89994032″,”term_text”:”DQ424925″DQ424925 (‘Enrichment and Thermophilic Mediator-Less Microbial Fuel Cell thermophilic microbial fuel cell enriched artificial wastewater clone 1B62′) , which showed an identity of 99.7% and an HSP coverage of 98.2%. The most frequently occurring keywords within the labels of environmental samples were ‘microbiota’ (4.1%), ‘microbi’ (4.1%), ‘intestin’ (4.0%), ‘mous’ (3.8%) and ‘compet, exploit, inflamm, salmonella, typhimurium’ (3.7%) (183 hits in total). The most frequently occurring keywords within the labels of environmental samples which yielded hits of a higher score than the highest scoring species were ‘microbi’ (8.6%), ‘thermophil’ (6.7%), ‘enrich’ (5.7%), ‘cell, fuel’ (5.3%) and ‘spring’ (3.6%) (21 hits in total), which seem to fit to the features known for C. nitroreducens.
Figure 1 shows the phylogenetic neighborhood of C. nitroreducens Yu37-1T in a 16S rRNA based Brefeldin_A tree. The two copies of the 16S rRNA gene in the genome differ by one nucleotide from each other any by up to one nucleotide from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AB364234″,”term_id”:”194072585″,”term_text”:”AB364234″AB364234). Figure 1 Phylogenetic tree highlighting the position of C.