One investigator per country reviewed all CRFs before its inclusion in the database. Patients were considered lost LY2157299 to follow-up if the last visit preceded the 9 months before
study closure. All national and, if necessary, local ethics committees approved the study, and all patients provided written informed consent to participate. Child-Pugh and Model for End-Stage Liver Disease (MELD) scores were calculated using the data at diagnosis of BCS, as previously reported.12, 13 The Rotterdam score was previously published to predict survival and is defined as follows: 1.27 × encephalopathy + 1.04 × ascites + 0.72 × prothrombin time + 0.004 × bilirubin (where ascites was scored as present “1” or absent “0”). The 5-year survival rate was 89% (95% confidence interval [CI]: 79-99) for class I, 74% (95% CI: 65-83) for class II, and 42% (95% CI: 28-56) for class III.9 The BCS-TIPS prognostic index score (TIPS-BCS PI score) was developed to predict OLT free survival in patients that received TIPS and is defined as follows: age (years) × 0.08 + bilirubin (mg/dL) × 0.16 + international normalized ratio (INR) × 0.63. The cutoff of 7 points EMD 1214063 ic50 had a sensitivity of 58%, a specificity of 99%, a positive predictive
value of 88%, and a negative predictive value of 96% for death or OLT 1 year after TIPS.6 Results are expressed as N (proportions) for categorical variables and as medians (range) for continuous 上海皓元医药股份有限公司 variables. Actuarial transplantation-free and intervention-free survival
rates were calculated by using Kaplan-Meier’s method. Uni- and multivariable Cox’s regression analysis was used to explore the association between different variables and prognosis. New prognostic scores were constructed by combining (in a linear equation) those variables independently associated with the event multiplied by their regression coefficients. To add potential advantages to these models, we did not include subjective parameters (e.g., presence or absence of hepatic encephalopathy; HE) or INR in patients that may have initiated anticoagulation that were integrated in the previously described scores. Statistical significance was defined as a P value less than 0.05. All statistical analyses were conducted with the PASW Statistics 18 program (SPSS, Inc., Chicago, IL). All 163 patients included in the previous EN-Vie study were eligible, and all centers, except one, that took part in the first study agreed to participate. Finally, 157 patients were included in the current study (Belgium, N = 5; France, N = 35; Germany, N = 14; Great Britain, N = 29; Italy, N = 18; Spain, N = 33; Switzerland, N = 4; The Netherlands, N = 19). Overall median follow-up of these 157 patients was 50 months (range, 0.1-74.0). Twenty-six patients (17%) were lost to follow-up after a median time of 25 months (range, 0.