Appl Environ Microbiol 2005, 71:8201–8206.PubMedCentralPubMedCrossRef
Competing interests The authors declare that they have no competing interests. Authors’ contributions PP carried out the collection of the pyrosequencing and patient data, contributed to the statistical analyses of these data sets and helped draft the manuscript. HJ coordinated the collection of the patient specific data and helped to draft the manuscript. AP undertook the culture based analyses of samples. JDP participated in the study design, culture based analyses and coordination and helped to draft the manuscript. CJS generated sequence information and contributed to the statistical analysis. AN contributed to the statistical analyses of these data sets and helped draft the manuscript. CL selleck compound participated in the design of the study
and performed the statistical analysis. DLS participated in the generation and analysis the sequence data. SPC conceived of the study, and participated in its design and coordination and drafted the manuscript. ADS conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.”
“Background The extensive use of antimicrobials during the last half century has promoted the evolution of antimicrobial resistance characteristics in pathogenic and opportunistic microorganisms [1, 2]. The SBI-0206965 manufacturer selective pressures induced by antimicrobial therapies have forced the acquisition and spread of a variety of antimicrobial resistance determinants. Resistance mutations may arise spontaneously or certain organisms may derive these from foreign DNA encountered at sites
of infection. Many organisms have steadily gained resistance due to their ability to uptake DNA from the surrounding before environment and incorporate it into their genome. For example, Falsetta  studied N. gonorrhoeae, which is naturally competent and gains resistance by using several systems of DNA uptake to acquire foreign DNA. At the same time, several strains actively release their DNA into the environment. Thus, resistance genes can come from self-organisms and non-self-organisms. In addition to the development of resistance, many pathogenic and opportunistic bacterial species utilize other strategies that enable them to evade clearance from their host, such as of the formation of biofilm structures that are recalcitrant to removal . Although the definition of a biofilm has fluctuated over the last 20 years, classically biofilms are defined as microorganisms that are irreversibly attached to a surface, which are PF 01367338 encased in a protective (often self-produced) matrix that may be composed of eDNA, exopolysaccharides, host material, shed membranes, etc. [5, 6]. These organisms tend to work cooperatively to ensure community survival, where some may forfeit active growth [7, 8].